LOCUS       40924_49317        3816 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD070, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3816)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3816)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    405..410
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    408..428
                     /note="GC; GC-adaptor for seamless assembly, flanking
                     antibiotic resistance markers or Escherichia coli origins 
                     to be assembled with a cyanobacterial replicon; synthetic"
     misc_feature    429..434
                     /label=NheI
                     /note="NheI"
     CDS             complement(574..1431)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1432..1503)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(1432..1501)
                     /note="Pbla; upstream region of the bla ORF including
                     promoter and RBS; derived from pUC19"
                     /regulatory_class="promoter"
     misc_feature    1505..1510
                     /label=AgeI
                     /note="AgeI"
     misc_feature    1511..1531
                     /note="C2G; GC-adaptor for seamless assembly, flanking 
                     antibiotic resistance markers, functional devices or custom
                     sequences; synthetic"
     misc_feature    1529..1534
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    1553..1558
                     /label=XbaI
                     /note="XbaI"
     primer_bind     complement(1595..1611)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1619..1635)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1643..1673)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1688..1709)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1997..2585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2759..3616)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3617..3721)
                     /label=AmpR promoter
     misc_feature    3747..3752
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgaggatatc gcgcgcgcgc
      421 gcgcggatgc tagcctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
      481 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
      541 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
      601 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
      661 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
      721 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
      781 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
      841 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
      901 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
      961 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1021 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1081 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1141 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     1201 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     1261 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     1321 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     1381 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     1441 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     1501 atgtaccggt atcccgccgc cgccgccgga tatcctcggt acccggggat cctctagagt
     1561 cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     1621 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     1681 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1741 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1801 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1861 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1921 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1981 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2041 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2101 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2161 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2221 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2281 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2341 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2401 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2461 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2521 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2581 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2641 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2701 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2761 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2821 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2881 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2941 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3001 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3061 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3121 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3181 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3241 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3301 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3361 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3421 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3481 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3541 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3601 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3661 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3721 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     3781 cctataaaaa taggcgtatc acgaggccct ttcgtc
//
